Spectroscopic Studies on the Interaction of Anti Cancer Rosemary With ctDNA

Abstract

Background: Some herbal anticancer agents have direct interactions with DNA. Objectives: The present study aimed to investigate the interaction of ctDNA with rosemary flowers. Materials and Methods: We used UV-Vis, fluorescence and circular dichroism (CD) spectroscopic techniques. Results: The absorption of DNA at 260 nm increased on addition of rosemary. Herb extract quench the fluorescence of EtBr bound to ctDNA. The Stern-Volmer constant (K) value for rosemary extract is 0.39 (mg/mL)-1. Finally, the CD spectra indicate that rosemary induces some conformational changes in ctDNA structure such as B to C-form transition. Conclusions: Our results illustrate that rosemary extracts interact with ctDNA through minor groove binding. This is one of the molecular mechanisms underlying anticancer effects of rosemary.