Spectroscopic Studies on the Interaction of Anti Cancer Rosemary With ctDNA
| Author | Seyedeh Elham Mostafavinia | en |
| Author | Reyhane Hoshyar | en |
| Orcid | Reyhane Hoshyar [0000-0001-7713-3897] | en |
| Issued Date | 2016-04-01 | en |
| Abstract | Background: Some herbal anticancer agents have direct interactions with DNA. Objectives: The present study aimed to investigate the interaction of ctDNA with rosemary flowers. Materials and Methods: We used UV-Vis, fluorescence and circular dichroism (CD) spectroscopic techniques. Results: The absorption of DNA at 260 nm increased on addition of rosemary. Herb extract quench the fluorescence of EtBr bound to ctDNA. The Stern-Volmer constant (K) value for rosemary extract is 0.39 (mg/mL)-1. Finally, the CD spectra indicate that rosemary induces some conformational changes in ctDNA structure such as B to C-form transition. Conclusions: Our results illustrate that rosemary extracts interact with ctDNA through minor groove binding. This is one of the molecular mechanisms underlying anticancer effects of rosemary. | en |
| DOI | https://doi.org/10.17795/gct-35638 | en |
| Keyword | Interaction | en |
| Keyword | DNA | en |
| Keyword | Rosemary | en |
| Keyword | Spectroscopic | en |
| Publisher | Brieflands | en |
| Title | Spectroscopic Studies on the Interaction of Anti Cancer Rosemary With ctDNA | en |
| Type | Research Article | en |
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